Journal: Pharmaceuticals

Article Title: Increased MAGE-C Family Gene Expression Levels as a Biomarker of Colon Cancer Through the Demethylation Mechanism

doi: 10.3390/ph17111447

Figure Lengend Snippet: Identification of MAGE-C gene transcripts in tissue specimens collected from male patients with adjacent NC and CC tissues. ( A ) The RT-PCR results for the MAGE-C family genes are shown on agarose gel pictures. The cDNAs were created from the total RNA taken from 20 tissue samples, 20 of which were NC and 20 of which were CC. To ensure the integrity of the cDNA samples, the expression of the ACTB gene was evaluated, yielding a band of approximately 553 bp. The primers for each gene were validated using cDNA obtained from testicular tissue. The official names of the individual genes along with their expected product sizes are shown to the left of the gel images. ( B ) qRT-PCR analysis of MAGE-C family gene expression was measured in NC and CC samples. This qRT-PCR quantified the mRNA expression of these genes in CC samples relative to their respective NC samples, with results normalized to GAPDH mRNA levels. The error bars in the figure indicate the standard error of the mean from three separate qRT-PCR experiments for each gene. Significance is indicated by ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0.0001. Abbreviations: NC (normal colon); CC (colon cancer).

Article Snippet: Additionally, to further validate the specificity of each MAGE-C primer, RT-PCR was conducted using cDNA synthesized from RNA extracted from human testis tissue (AM7972; Thermo Fisher Scientific) known to express MAGE-C genes.

Techniques: Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Expressing, Quantitative RT-PCR, Gene Expression